Journal: Journal of Translational Autoimmunity

Article Title: T-cell immunity in the experimental autoimmune vasculitis rat model

doi: 10.1016/j.jtauto.2025.100305

Figure Lengend Snippet: The anti-IL-17A antibody clone eBio17CK15A5 inhibits the biological activity of IL- 17A in vitro . (A,B) The rat renal tubular cell line (NRK52E cells) was stimulated with IL-17A or TNFα+IL-17A in absence or presence of neutralizing IL-17A antibody (n = 4 independent experiments). Unstimulated cells served as control. The gene expression of the IL-17A target genes CCL20 and CXCL1 served as readout. Stimulation with IL-17A induced the gene expression of CCL20 and CXCL1 already after 24 h. Co-stimulation with TNFα further increased CCL20 and CXCL1 transcripts. The presence of IL-17A antibody led in all cases to a reduction of CCL20 and CXCL1 transcripts confirming the inhibitory activity of the antibody.

Article Snippet: NRK52E cells (Leibniz Institute, DSMZ, Braunschweig, Germany) were cultured in complete DMEM supplemented with 5 % fetal calf serum (Biowest) and 1 % penicillin/streptomycin. (Thermo Fisher Scientific).

Techniques: Activity Assay, In Vitro, Control, Gene Expression

Journal: Acta Pharmaceutica Sinica. B

Article Title: Targeting renal tubular epithelial cells via a dual functionalized oligosaccharide self-assembly in the management of acute and chronic kidney diseases

doi: 10.1016/j.apsb.2025.09.017

Figure Lengend Snippet: Schematic illustration of an active and passive dual-targeted delivery system (COA-SA/CLT-siSnail) for the codelivery of small molecule drugs and therapeutic nucleic acids to RTECs in the treatment of renal disease.

Article Snippet: Rat renal tubular epithelial cells NRK-52E, rat fibroblasts NRK-49F, and human proximal tubular epithelial cells HK-2 were purchased from American Type Culture Collection (Manassas, VA, USA), mouse renal tubular epithelial cells TCMK-1 were obtained from Dr. Li Yanping’s laboratory, and maintained in Dulbecco’s modified Eagle’s medium (DMEM) with high glucose supplemented with 10% ( v / v ) fetal bovine serum (FBS), streptomycin (100 μg/mL) and penicillin (100 U/mL) (Thermo Fisher, USA).

Techniques:

Journal: Acta Pharmaceutica Sinica. B

Article Title: Targeting renal tubular epithelial cells via a dual functionalized oligosaccharide self-assembly in the management of acute and chronic kidney diseases

doi: 10.1016/j.apsb.2025.09.017

Figure Lengend Snippet: Cellular uptake and internalization pathway of COA-SA/Cy5-siSnail micelles. (A) LSCM analysis cellular uptake of free Cy5-siSnail, COA-SA/Cy5-siSnail, Lipo6000/Cy5-siSnail in NRK-52E and HK-2 cells. Scale bars, 20 μm. (B) Flow cytometry analysis shows a positive rate of different preparations in RTECs. (C) Internalization of COA-SA/siSnail micelles in NRK-52E and HK-2 cells pretreated with 4 °C, methyl- β -cyclodextrin (M- β -CD), nystatin (Nys), 5-( N , N -dimethyl)-amiloride hydrochloride (DMA) and chlorpromazine (Chlo), respectively. ∗∗∗∗ P < 0.0001 vs . control. (D) Internalization of COA-SA/siSnail micelles in NRK-52E and HK-2 cells pretreated with EDTA, Gentamicin, Megalin antibody, and chitosan oligosaccharide (COS), respectively. ∗ P < 0.05; ∗∗ P < 0.01; ∗∗∗ P < 0.001; ∗∗∗∗ P < 0.0001 vs . control. Data represent mean ± SD ( n = 3). (E) LSCM shows the endosome/lysosome escape of COA-SA/Cy5-siSnail micelles in NRK-52E and HK-2 cells. Scale bars, 10 μm. (F) Inhibitory effects of COA-SA/CLT-siSnail micelles on the viability of RTECs. Data represent mean ± SD ( n = 6).

Article Snippet: Rat renal tubular epithelial cells NRK-52E, rat fibroblasts NRK-49F, and human proximal tubular epithelial cells HK-2 were purchased from American Type Culture Collection (Manassas, VA, USA), mouse renal tubular epithelial cells TCMK-1 were obtained from Dr. Li Yanping’s laboratory, and maintained in Dulbecco’s modified Eagle’s medium (DMEM) with high glucose supplemented with 10% ( v / v ) fetal bovine serum (FBS), streptomycin (100 μg/mL) and penicillin (100 U/mL) (Thermo Fisher, USA).

Techniques: Flow Cytometry, Control